Abstract Basic fibroblast growth factor (bFGF), a protein, plays a key role in wound healing and blood vessel regeneration.However, bFGF is easily degraded in biologic systems.Mesoporous silica nanoparticles (MSNs) with well-tailored porous structure have been used for hosting guest molecules for drug delivery.Here, we report an in 7gm pravana situ route to load bFGF in MSNs for a prolonged release.
The average diameter (d) of bFGF-loaded MSNs is 57 ± 8 nm produced by a water-in-oil microemulsion method.The in vitro releasing profile of bFGF from MSNs in phosphate buffer saline has been monitored for 20 days through a colorimetric enzyme linked immunosorbent assay.The loading efficiency of bFGF in MSNs is estimated at 72.5 ± 3%.
In addition, the cytotoxicity test indicates that the MSNs are not toxic, even at a concentration of g35 coupe fender 50 μg/mL.It is expected that the in situ loading method makes the MSNs a new delivery system to deliver protein drugs, e.g.growth factors, to help blood vessel regeneration and potentiate greater angiogenesis.